Search results for "Culture condition"
showing 2 items of 2 documents
Comparative morphometrics of sharpsnout seabream (Diplodus puntazzo Cetti, 1777), reared in different conditions
1999
The same genetic stock of sharpsnout seabream (Diplodus puntazzo), cultured in different conditions (lot A, monoculture tank; lot B, monoculture offshore cage; lot C, polyculture tank), was analysed using geometric morphometrics in order to detect changes in shape. Rearing in the offshore cage (lot B) had a significant effect on shape variation when compared to rearing in the two enclosed tanks (A and C). Fish from the tanks show a higher frequency (43.7% in lot A and 37.5% in lot C) of the so-called ‘goitred’ and ‘dorsal flat’ forms, their deformations shown by displacements in the corresponding landmarks of the infraopercular and dorsal areas. In contrast, the so-called ‘belly’ fish were …
Fetal Calf Serum-Free Generation of Functionally Active Murine Dendritic Cells Suitable for In Vivo Therapeutic Approaches
2000
Standard protocols to generate mouse dendritic cells (DC) generally use culture medium supplemented with fetal calf serum; however, reinjection in vivo of DC cultured in fetal calf serum results in priming to xenogeneic proteins that clearly limits the use of such DC. We therefore established a fetal calf serum-free culture system for the generation of murine DC from bone marrow precursors. DC can be generated fetal calf serum-free using RPMI supplemented with 1.5% syngeneic mouse serum. Although the yield of DC grown under fetal calf serum-free conditions was somewhat lower than that of the standard culture, large numbers of DC could be generated without the exposure to xenogeneic proteins…